Map of the myeov–cyclin D1 region at 11q13, constructed by fiber FISH.

The map is an extended version of the previously reported fiber-FISH map.26 The scale was based on restriction mapping of the cosmids cos 6.22, cos 3.62, and cos 3.91, which together were used as internal standard of 113.4 kilobase (kb).35 All available probes in the region are shown in the top part of the figure (see “Materials and methods”). Myeov was detected using a pool of the 3 subclones. The transcriptional orientation of themyeov and cyclin D1 genes is indicated with horizontal arrows. Hybridization of YAC A7D7* revealed an internal deletion that is apparent as 2 separate, discontinuous signals. The bottom part shows the localization of translocation breakpoints in 7 MM cell lines and 5 mantle cell lymphomas (p11, p14, p26, p29, and p252) as determined by fiber FISH. Mapping of the mantle cell lymphoma breakpoints has also been described previously.26