Northern blot analysis of the 3 MM cell lines that show high expression of both myeov and cyclin D1 (A) and other MM cell lines (B).

Ten micrograms of total RNA isolated from the indicated cell lines was submitted to Northern transfer. The filters were independently hybridized to a ³²P-labeled myeov cDNA insert (upper panel), and a cyclin D1 cDNA insert (middle panel). 28S and 18S ribosomal RNA were used as molecular weight markers. An ethidium bromide–stained gel indicates the amount of RNA loaded into each lane (lower panel).