Overview of the results of fiber-FISH mapping of t(11;14) breakpoints in 7 MM cell lines.

Red and green bars represent probes detected with Texas Red and FITC, respectively; yellow indicates areas of overlapping Texas Red– and FITC-stained probes. The top 2 color bar codes show the normal IgH/14q32 and BCL-1/11q13 loci. For each cell line, both 14q+ and 11q-fusion products are shown, and the myeov andcyclin D1 genes are indicated with a small arrow and a larger arrowhead, respectively. The position of the Eμ enhancer, as determined using an Eμ-specific probe in separate experiments, is indicated with a black circle. The position of the 3′ Eα enhancers was not determined by hybridization with a specific probe, but was derived from the presence of the Sα/Cα plasmid probe. Where 2 Sα/Cα probe signals are present, the enhancers associated with upstream signals and downstream signals are labeled Eα1 and Eα2, respectively.