Surface phenotype of fresh or activated DC2 from G-CSF-treated donors.

DC2 from G-CSF-treated donors were purified as shown in Figure 3. DC2 were tested for the expression of CD40, CD80, and CD86 either fresh (upper panels) or after 48 hours pre-activation in vitro by GM-CSF, IL-3, and TNF-α (lower panels). IL-3Rα (CD123) expression confirms that the DC2 population was not contaminated with other cell types that might have expanded during in vitro culture. Fluorescence signal of cells stained with the specific antibody is shown in solid black, whereas the background of cells stained with an isotype-matched antibody of irrelevant specificity is shown in white with a black contour.