Cytokine secreted by CD4+/CD45RA+ T cells after stimulation with activated, allogeneic DC1 or DC2.

Allogeneic DC1 or DC2 were purified from G-CSF-treated donors, as shown in Figure 3, and activated for 48 hours in vitro with GM-CSF, IL-3, and TNF-α. CD4⁺CD45RA⁺ naive T cells were purified by immunomagnetic beads, as described in “Materials and Methods.” Purified CD4+/CD45RA+ T cells were stimulated by pre-activated DC1 or DC2 for 6 days. Then cells were restimulated with PMA plus ionomycin. Supernatants were harvested after 48 hours and frozen until analyzed by ELISA for the presence of IFN-γ, IL-10, and IL-4. Results are representative of 3 identical experiments showing similar results.