Expression of PLSCR1 in HT1080 cells.

Western blotting for PLSCR1 expressed in HT1080 (left panel) and U3A (right panel) cells after treatment with 1000 IU/mL IFN-α2a under conditions of Figure 1 was performed with mab 4D2 (see “Materials and methods”). The IFN-induced increase in PLSCR1 antigen detected in HT1080 (normalized to t = 0) was 1.5-fold (t = 3 hours), 4.3-fold (t = 6 hours), 8.3-fold (t = 18 hours), and 6.8-fold (t = 24 hours). In U3A, the increase was 1.4-fold (t = 3 hours), 1.3-fold (t = 6 hours), 1.3-fold (t = 18 hours), and 1.2-fold (t = 24 hours). Data of single experiment, representative of 3 so performed.