SDF-1 stimulation induces tyrosine phosphorylation of PLC-γ in CTS cells. CTS cells were serum-starved and stimulated with SDF-1α for the indicated times. Total cell lysates were immunoprecipitated with anti-PLC-γ antibody, and subjected to serial immunoblotting with anti-PY antibody (4G10) (upper panel) and anti-PLC-γ antibody (lower panel). The increase in tyrosine phosphorylation of PLC-γ is indicated (by fold increase) based on the densitometry values.