Association of p130 Cas with Crk or Crk-L on stimulation with SDF-1 in CTS cells. Total cell lysates from unstimulated or SDF-1α–stimulated CTS cells were immunoprecipitated with anti-Crk antibody (A) or anti-Crk-L antibody (B). The immune complexes were resolved on 10% SDS-PAGE and immunoblotted with anti-p130 Cas antibody (A and B, upper panels). The immunoblots were stripped and reblotted with anti-Crk (A, lower panel) or anti-Crk-L antibody (B, lower panel). TCL represents total cell lysates. The changes in the association of Crk (A) or Crk-L (B) with p130 are indicated (by fold increase) based on the densitometry values.