Wortmannin or GF109203X but not PD98059 inhibits SDF-1–-induced migration of CTS cells. CTS cells were treated with either carrier (DMSO) alone, various concentrations of wortmannin (A), GF109203X (B), or PD98059 (C) for 45 minutes. The cell migration in response to SDF-1α (20 nmol/L) was measured in a chemotactic assay as described in “Materials and methods.” Cell migration is shown as the percentage of cell input. The data shown represent the mean ± SD of 3 separate experiments.