Expression of GTPases, RhoA and Rac2, in neutrophils from healthy individuals and SCN patients.

Neutrophil lysates (50 μg protein each) were separated on a 8% SDS-PAGE, blotted onto nitrocellulose membranes, and detected with anti-RhoA or anti-Rac2 antibodies. These results were representative for 6 independent experiments with cells from 3 SCN patients, 3 G-CSF-treated individuals, and 6 untreated healthy individuals. N indicates normal neutrophils; SCN, patients' neutrophils; and d5, in vivo-stimulated neutrophils from a healthy donor.