Expression of the PRV-1 protein in granulocytes from a patient with PV.

Granulocytes were isolated from peripheral blood to more than 98% purity. Total cell extracts were prepared and subjected to SDS-PAGE. The proteins were blotted onto a nylon membrane and decorated with either the antibody raised against the C-terminal peptide (amino acids 368-383, lane 2) or the antibody raised against the N-terminal peptide (amino acids 13-25, lane 3). Immune complexes were detected by decoration with peroxidase-conjugated secondary antibody and visualized by chemoluminescence. Lane 1 shows a molecular size marker. The film was exposed for 5 seconds.