Expression of PRV-1 in other MPDs.

Mononuclear cells were isolated from peripheral blood of patients with CML (A) or AML (D). Granulocytes were isolated from peripheral blood of patients with ET (B), IMF (C), secondary erythrocytosis (E), PV (A, B, D, and E) or healthy volunteers (A and D). Total RNA was extracted and Northern blots prepared using 10 μg RNA. (Top) The membrane was probed with a 1.1-kb fragment of the PRV-1 cDNA. (Bottom) The membrane was stripped and reprobed with a 1.2-kb fragment of the human β-actin cDNA.