PML is required for the NB localization of SUMO-1, Sp100, Daxx, CBP, and ISG20.

Primary MEFs were grown on chambered slides before immunofluorescence analysis. MEFs were double-labeled for PML and (A) SUMO-1, (B) Sp100, and (C) Daxx. (D) The localization of endogenous CBP in PML^+/+ and PML^−/− MEFs. (E) The localization of transfected HA-ISG20 in PML^+/+ and PML^−/− MEFs. Primary cells were transfected with HA-ISG20. After 24 hours, cells were harvested and stained for the HA epitope. Representative confocal micrographs are shown, with the respective immunofluorescent colors (PML, red; SUMO-1/Sp100/Daxx/CBP/HA-ISG20, green; DAPI, blue) labeled in the lower corners of each image. Colocalization is reflected by the yellow color. Bar: 5 μm.