Transfected PML in PML^−/− cells recruits Daxx, SUMO-1, and Sp100 to the NBs.

PML^−/− keratinocytes were transfected with pSG5-PML alone or cotransfected with pSG5-PML and pEGFP-Sp100. After 24 hours, cells were harvested and cytospun onto glass slides for immunofluorescence staining. Representative confocal micrographs are shown (PML, red; SUMO-1/Daxx/GFP-SP100, green; DAPI, blue). The yellow color reflects colocalization. The arrow points to a cell transfected with GFP-Sp100 only. This represents the localization pattern of transfected Sp100 in PML^−/− keratinocytes where this protein aggregates in large patches. Bar: 5 μm.