Fig. 1.
HMEC-1 cells adhere to factor XIIIa in a dose-dependent manner.
Cell adhesion assays were conducted using immobilized thrombin-activated factor XIIIa. HMEC-1 cells were allowed to attach for 40 minutes at 37°C in 5% CO2 to wells coated with the indicated concentrations of factor XIIIa. Adherent HMEC-1 cells were fixed, stained with toluidine blue, solubilized with 2% SDS, and assayed for absorbance at 650 nm. Background binding to uncoated wells was subtracted from each value. Each point represents the mean of 6 determinations ± SD.
