Fig. 3.
HMEC-1 cell adhesion to factor XIIIa is dependent on particular divalent cations.
Cell adhesion assays were conducted in Hank's balanced salt solution in the presence of the indicated concentrations of CaCl2(black bars), MgCl2 (gray bars), or MnCl2(crosshatched bars). HMEC-1 cells were allowed to attach for 40 minutes at 37°C to wells coated with 15 μg/mL thrombin-activated factor XIIIa. Adherent HMEC-1 cells were fixed, stained with toluidine blue, solubilized with 2% SDS, and assayed for absorbance at 650 nm. Background binding to uncoated wells was subtracted from each value. Each bar represents the mean of 6 determinations ± SD.
