SDS polyacrylamide gel electrophoresis of urea-treated and untreated γA/γA and γA/γ′ fibrinogen.

Fibrinogen samples (5 μg) were run on a 10% polyacrylamide gel under reducing conditions and stained with Coomassie brilliant blue. Lane 1, molecular weight standards; lane 2, untreated γA/γA fibrinogen; lane 3, urea-treated γA/γA fibrinogen; lane 4, untreated γA/γ′ fibrinogen; and lane 5, urea-treated γA/γ′ fibrinogen.