Fig. 2.
Fig. 2. Characterization of the IGH rearrangements in SKI-DLCL-1 by PFGE. / High molecular weight DNA from peripheral blood lymphocytes (lane 1) and SKI-DLCL-1 cells (lane 2) was digested with NotI and resolved through PFGE with a window of resolution of 50 kb to 1500 kb (A-E) or 25 to 500 kb (F, G). The identical blot was sequentially hybridized with a series of probes, as indicated in A to E. A second blot was used (F, G) for hybridization by the indicated probes. The samples are as follows: lane 1, PBL; lane 2, ascitic fluid; lane 3, SKI-DLCL-1. Complete removal of the different probes after stripping was confirmed by exposure to film. ZNR, zone of no resolution.

Characterization of the IGH rearrangements in SKI-DLCL-1 by PFGE.

High molecular weight DNA from peripheral blood lymphocytes (lane 1) and SKI-DLCL-1 cells (lane 2) was digested with NotI and resolved through PFGE with a window of resolution of 50 kb to 1500 kb (A-E) or 25 to 500 kb (F, G). The identical blot was sequentially hybridized with a series of probes, as indicated in A to E. A second blot was used (F, G) for hybridization by the indicated probes. The samples are as follows: lane 1, PBL; lane 2, ascitic fluid; lane 3, SKI-DLCL-1. Complete removal of the different probes after stripping was confirmed by exposure to film. ZNR, zone of no resolution.

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