Restriction map of cosmid S7D.

Cosmid S7D was subcloned as a BamHI sublibrary. Sequence of the ends of clone B61 was found to be colinear with YAC MNG1 and was homologous to the thrombospondin 3 gene. MUC1 gene extends from clone B22 to clone B109, which spans the breakpoint. Two boxes indicate the position of the fragments used as probes (RsaI 3.5-kb andBstxI 1.1-kb fragment). Restriction sites are indicated: N,NotI; B, BamHI; E, EcoRI; M,MluI; Bss, BssHII.