Expression of loci within 90 kb of the t(1;14) translocation breakpoint.

(A) Physical map of the cluster of genes identified near the Gaucher disease locus based on sequence data (map based on previously published data in Adolph et al18). (B) Expression of the cluster of genes upstream of the breakpoint by Northern blot analysis included the following loci: CLK2, propin, cote1, GBA, metaxin, and thrombospondin 3. Total RNA (10 μg) was electrophoresed on a 1% formaldehyde agarose gel. 28S was used as a control for RNA loading. The size of the expected transcripts is indicated. Lanes 1 to 8 represent different cell lines: lane 1, Hela; lane 2, UC726/G; lane 3, SKI-DLCL-1; lane 4, Daudi; lane 5, OCI-LY8; lane 6, FL318; lane 7, Raji; lane 8, Molt-4.