NPM-RAR interacts with the co-repressor SMRTe and the co-activator RAC3. (A) Autoradiographs show the in vitro translated³⁵S-methionine labeled proteins used in the Far-Western blots. (B) Far-Western blots show the interaction of in vitro translated RARα, NPM-RAR, and PML-RAR fusion proteins with GST-SMRTe (AA 1993-2507) and GST-RAC3 ID (AA 613-752) in the absence (−RA) or presence (+ RA) of 1 μM ATRA. The specific activity of all probes were similar and the exposure time identical in 1 film. (C) Far-Western blot analyses show the interaction of wild-type NPM, PML-1, and PLZF with GST-SMRTe (1993-2507).