Changes in cell viability during the culture with or without Ub.

(A) HL-60 and KT-3 cells were resuspended in ASF103 and ASF103 containing 1 ng/mL rhIL-6, respectively, and cultured in the presence or absence of 100 μg/mL Ub for 72 hours. Changes in cell viability were quantitated by trypan blue dye exclusion method at the times indicated. The results are shown as the mean ± SD of triplicate cultures. (B) Flow cytometric analyses of HL-60 and KT-3 cells during the culture with or without Ub. The DNA content of the cultured cells was examined by PI staining and analyzed on FACSort.