FVIII antigen expression in transgenic epidermis.

Immunostaining for factor VIII expression using an antihuman factor VIII antibody (A-C) showed an intense band of staining confined to the suprabasal epidermis in transgenic skin (B), whereas nontransgenic skin (A) and transgenic skin treated only with secondary antibodies (C) show no epidermal staining for factor VIII. Immunostaining for vWF using an antihuman vWF antibody that cross-reacts with murine vWF revealed focal signals confined to the dermal vascular endothelium in both transgenic (D) and nontransgenic (E) skin. Skin from a vWF knockout mouse (F) was used as a negative control and showed no positive signal.