RT-PCR analysis of pinvVIIILA expression in transgenic mice.

RT-PCR analysis of several tissues from a pinvVIIILA mouse is shown. The primer pair depicted in Figure 1 gives a fully spliced, message-specific product of 210 base pairs. There is evidence of transgene expression in skin and other tissues that have stratified squamous epithelia. Tissues that have no stratified squamous epithelia show no transgene expression. Control RT-PCR reactions for RNA integrity used primers specific for mouse glyceraldehyde phosphate dehydrogenase (GAPDH) message (bottom panel).