Factor VIII activity analysis in transgenic mice.

Factor VIII activity in samples of transgenic mouse plasma was measured using the COAMATIC assay. A standard curve for factor VIII activity was generated from pooled plasma of normal mice. Knockout mouse plasma was used to generate a baseline point (less than 1% of normal activity) for the standard curve. The average factor VIII activity for each line compared with normal mice is displayed, and the range of activity observed is shown in parentheses. The number of mice assayed from each line is listed in the bottom row.