Analysis of CD45RA and CD62L expression versus CD95 expression in both CD4 and CD8 subsets.

Freshly isolated PBMCs were stained with the following combinations of FITC-, PE-, and CyChrome-conjugated mAbs: CD95, CD45RA or CD62L, and CD4 or CD8. Analysis of CD45RA and CD62L expressions (Y axis) versus the CD95 expression (X axis) was carried out using lymphocytes from the same donors of Figure 1. (A) Within CD4 T cells, all CD95⁻ cells (square box) were CD45RA⁺ and CD62L⁺ in both young and old donors. (B) Similarly, all CD8⁺CD95⁻ cells (square boxes) were CD45RA⁺ and CD62L⁺. It is important to note that in both CD4 and CD8 cells, the percentages of CD95⁻ T cells were substantially identical to those of the double-positive CD45RA⁺CD62L⁺ T cells shown in Figure 1.