Expression of the N-terminal HA-tagged and the C-terminalmyc-epitope–tagged human EDG-6 receptor . (A, C) Intact and (B, D) permeabilized HEK293 cells transfected with C-terminalmyc-epitope–tagged human EDG-6 (A, B) or with N-terminal HA-tagged human EDG-6 (C, D). FACS analysis was performed as described in “Materials and methods.” Grey areas indicate the signal without the first antibody (A, B) or autofluorescence of unstained cells (C, D), respectively.