Fig. 4.
SPP binds to EDG-6 with high affinity . HEK293 cells were transfected with EDG-6 and specific binding of [32P]SPP at the indicated concentrations was determined. “Specific binding” is binding in the absence of unlabeled SPP minus binding in the presence of excess unlabeled SPP. Results are means ± SD of triplicate determinations. The binding curve and the KD (63 nmol/L) were calculated using Deltagraph for Macintosh.