Effects of RAR-, RARβ-, and RARγ-selective retinoids on SCF-dependent mast cell growth.
(A) Dose response to RARα-selective retinoid (Ro 40-6055), RARβ-selective retinoid (Ro 19-0645), or RARγ-selective retinoid (Ro 44-4753) of mast cell growth supported by SCF. One × 104 10-week cultured mast cells were incubated in wells containing SCF at 100 ng/mL with various concentrations of Ro 40-6055, Ro 19-0645, Ro 44-4753, or ATRA. After 2 weeks, the viable cells were enumerated. The results shown are from 1 representative experiment of 3. Similar results were obtained in the other 2 experiments. Significantly different from SCF alone (*P < .0001). (B) No cooperative effects of Ro 40-6055 at concentrations of 10−11 mol/L to 10−7 mol/L and Ro 19-0645 at concentrations of 10−9 mol/L to 10−7 mol/L on the growth of mast cells supported by SCF. Significantly different from no Ro 19-0645 (*P < .0001).
