Expression of Notch-1 and Notch-2 by human bone marrow cells and peripheral blood.

(A) Fluorescence histograms of human bone marrow cells stained with anti–Notch-1 and -2 antibodies and CD34, or CD34 and the absence of other antigens associated with lineage commitment. The x-axis represents log fluorescence intensity and the y-axis represents cell number. The solid line represents staining with anti–Notch-1 and -2 antibodies, and the dashed line represents staining with an isotype-matched control antibody of irrelevant specificity. (B) Notch-1 and -2 expression by human bone marrow cells committed to monocytic (CD14), granulocytic (CD15), and erythroid (glycophorin A) lineages. The x-axis represents log fluorescence intensity and the y-axis represents cell number. The solid line represents staining with anti–Notch 1-and 2-antibodies, and the dashed line represents staining with an isotype-matched control antibody. (C) Notch-1 and -2 expression by human peripheral blood cells. Fluorescence histograms for 2 color studies gated on cells committed to monocytic (CD14), granulocytic (CD15), T-cell (CD4, 8), or B-cell (CD20) lineages are shown. The x-axis represents log fluorescence intensity and the y-axis represents cell number. The solid line represents staining with anti–Notch-1 and -2 antibodies, and the dashed line represents staining with an isotype-matched control antibody.