Presentation of Delta^ext-myc.

(A) A schematic diagram of the truncated form of the extracellular domain of Delta-1, Delta^ext-myc. The construct contains the extracellular domain of Delta, including the signal peptide (SP), conserved DSL domain, EGF repeats, and 6 myc tag epitopes. (B) A schematic diagram of the presentation of Delta^ext-myc. Each panel represents a mode of presentation of Delta^ext-myc. In Panel A, Delta^ext-myc is presented in immobilized form, bound to 9E10 F(ab′)2 antibody fragments that is bound to the plastic. In Panel B, Delta^ext-myc is presented in the absence of antibody fragments (see “Materials and methods”).