Induction of apoptosis in monocytes by Delta^ext-myc in the presence of M-CSF.

Ten thousand monocytes were cultured with 10% FCS IMDM containing M-CSF (10 ng/mL) in the presence of Delta^ext-myc (1 μg/mL) or as a control, control material from nontransfected NSO cells. All wells were coated with anti-myc 9E10 F(ab′)₂ fragments. Cells were harvested at 12, 24, and 48 hours, washed with binding buffer, and incubated in binding buffer containing annexin V-FITC and PI. The x-axis represents log fluorescence intensity with PI staining; the y-axis represents log fluorescence intensity with annexin V staining.