Fig. 1.
Vascular endothelial growth factor (VEGF) expression in myeloma cells.
VEGF transcripts in human myeloma cell lines (lanes 2-5) and in CD38high/CD138+ or CD38high/CD56+ sorted myeloma cells from the bone marrow of patients with multiple myeloma (MM 1-4) were analyzed by RT-PCR. The primers used allowed the amplification of the known splice variants of VEGF. Only the two lower molecular weight isoforms VEGF165 (648 base pairs [bp]) and VEGF121 (516 bp) were detected. Peripheral blood B lymphocytes from healthy volunteers served as controls (PBB-L 1-4). Glyceraldehyde-3-phosphate dehydrogenase (347 bp) was used as a control for RNA integrity. M = size marker; C = PCR control.