Effect of vascular endothelial growth factor (VEGF) on interleukin-6 (IL-6) secretion by bone marrow stromal cells (BMSCs).

IL-6 concentrations were determined in culture supernatants of BMSCs after stimulation with 2 (•), 10 (▾), or 50 (▪) ng/mL of VEGF165 (A) or VEGF121 (B) for 6, 24, and 48 hours, respectively; (▴), unstimulated controls. Data represent median values and interquartile ranges of stimulation experiments performed in triplicates in three different BMSC cultures. Analysis of significance for overall group differences was performed by the Kruskal-Wallis test (P < .001). The multiple comparisons' criterion was employed to identify differences between groups (10 ng/mL vs controls,P < .01; 50 ng/mL vs controls, P < .001; 50 ng/mL vs 2 ng/mL, P < .001 for all time periods).