Fig. 9.
Effect of interleukin-6 (IL-6) on vascular endothelial growth factor (VEGF) expression in sorted marrow myeloma cells from patients (n = 12).
(A) VEGF concentrations in supernatants of myeloma cell cultures after exposure to 0 and 10 ng/mL IL-6 for 72 hours, presented as pg/mL corrected for 106 cells. P < .005 for difference between unstimulated and stimulated myeloma cells (Wilcoxon test). (B) VEGF transcription in IL-6-stimulated myeloma cells from patients. RT-PCR analysis of VEGF transcripts after exposure to 0 or 10 ng/mL IL-6 for 72 hours. Representative examples of three patients with increased VEGF messenger RNA levels after IL-6 stimulation are shown. Corresponding patient numbers (UPN) are indicated in panels A and B.
