Chemokine secretion in culture supernatants of IL-2–stimulated PBMCs.
Cultures were established from a mono–HTLV-II–infected patient (PR-1), whereas the bars indicate the range of chemokine concentrations of PR-2 and PR-3 (A). Similar cultures were performed with PBMCs of the HIV-1/HTLV-II–coinfected patient PR-7, with the bars indicating the range of PR-8, (B); of HIV-1/HTLV-II–coinfected patients PR-10, with bars indicating the chemokine levels observed with cells of PR-9 (C); and of HIV-1/HTLV-II–coinfected patients PR-12 (D). Cells were seeded at 1 × 105 cells per well and culture supernatants were harvested at regular intervals and then tested for chemokine concentrations and HIV-1 or HTLV-II p24 Gag Ags. Results are representative of 3 independent experiments.
