Fig. 2.
Expression of P230 Bcr-Abl in primary mouse bone marrow cells leads to tyrosine phosphorylation of cellular proteins.
Whole cell lysates were prepared from cytokine and serum-starved primary mouse bone marrow cells by boiling in 2 × sample buffer. Lysates were analyzed by SDS-PAGE and Western blotting with antibodies against (A) Abl or (B) phosphotyrosine.