Fig. 7.
p230-expressing primary mouse bone marrow cells require cytokines for optimal growth.
GFP-selected, control or Bcr-Abl–expressing primary mouse bone marrow cells were expanded for 10 days with cytokines in the absence of autologous stroma then split into duplicate cultures with (upper panels) and without (lower panels) cytokines. Twenty-four hours later, cultures were incubated in bromodeoxyuridine and analyzed for S-phase fraction.