Fig. 5.
p53 stabilization is increased by lactacystin treatment of PHA–PBMC and MT-2 cells.
Normal PHA–PBMC and MT-2 cells were treated with lactacystin, and the cell lysates were immunoprecipitated with the N-20 antibody. The blots were immunoblotted first with the N-20 antibody (A) and, after stripping, with the FL-393 antibody (B). + and − refer to the presence or absence of the drugs, respectively.