Characterization of the mouse RAG-2 promoter region.

(A) Mapping of the transcription initiation site of mouse RAG-2by 5′ RACE. The darkened circle denotes the transcription initiation sites of independent cDNA clones characterized by DNA sequence analysis. The major transcription initiation site (+1) and the first exon (boxed) are noted. An arrow indicates the 5′ end of the reported mouse RAG-2 cDNA.1 (B) Alignment of the mouse RAG-2 promoter region with that of the human RAG-2 promoter region. The major transcription initiation site (+1) is indicated by an arrow. The asterisk denotes the identical nucleotide (indicated by a capital letter). Some spaces (dotted lines) are inserted to produce maximal matching. Initiator sequence-like sequence is underlined. Boxed sequences indicate the −80/−17 fragment used as a probe for EMSA in Figures 4 and 5.