Possible involvement of Pax-5 and GATA-3 in mouse RAG-2 promoter activity.

Luciferase constructs were transfected together with pSRα-LacZ reference plasmids into 5 × 10⁶ B-lineage cells (18.8.1) or T-lineage cells (LSB11-1) and analyzed for activity as shown in Figure 2. Four luciferase constructs were used: without a promoter, with the wild type −86/+147 RAG-2 promoter (WT), or with the promoter containing a nucleotide alteration in either the Pax-5 binding site (M2) (Figure 4C) or GATA binding site (Gm) (Figure6A). Representative data of 3 independent experiments are shown.