American Society of Hematology

Fig. 2.

$Fig. 2. Ex vivo expanded hematopoietic cells are actively cycling. / The proportion of nucleated cells in G0/G1 or S/G2/M phases of the cell cycle was determined by HO staining of (A) normal murine BM, (B) Sca-1+c-kit+Lin+ cells isolated from day 1 post–5-FU BM, or (C) these cells' progeny generated after ex vivo expansion. Numbers in each panel represent the mean ± SEM of 3-9 measurements per population. The horizontal bars (C) represent the gates used for sorting expanded cells into G0/G1 or S/G2/M fractions prior to transplantation.$

Ex vivo expanded hematopoietic cells are actively cycling.

The proportion of nucleated cells in G₀/G₁ or S/G₂/M phases of the cell cycle was determined by HO staining of (A) normal murine BM, (B) Sca-1⁺c-kit⁺Lin⁺ cells isolated from day 1 post–5-FU BM, or (C) these cells' progeny generated after ex vivo expansion. Numbers in each panel represent the mean ± SEM of 3-9 measurements per population. The horizontal bars (C) represent the gates used for sorting expanded cells into G₀/G₁ or S/G₂/M fractions prior to transplantation.

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