AcSDKP inhibits the cycling of hematopoietic progenitors generated in vitro.
Sca-1+c-kit+Lin− cells were cultured (2 × 103/mL) in serum-free medium containing IL-6, IL-11, G-CSF, SCF, FLK, and TPO. Except for control cultures, AcSDKP was added daily to duplicate wells on days 1-6. Shown are (A) the mean ± SEM number of nucleated cells counted after 7 days and (B) the proportion of cycling CFCs in each culture assayed by plating cells in methylcellulose-based medium before or after exposure to HU, as described in the “Materials and methods.” The reduction in total nucleated cell production and CFC cycling in cultures containing 10−12 mol/L AcSDKP was statistically significant. (*indicates P < .01, and **indicatesP < .03.) The data given are pooled from 3 experiments.
