Fig. 1.
ESI-MS spectrum of a representative HPLC fraction.
The spectrum shows 3 different charge states [M+2H]2+, [M+3H]3+, and [M+4H]4+ of HNP-1 (1721.3, 1148.1, 861.3) and HNP-2 (1685.8, 1124.6, 843.5), respectively. This indicates that these peptides have full lengths of 30 and 29 amino acids, respectively.