Fig. 3.
Defensin isolate as competitor for the HLA-DR1 binder AMCA-HA 306-18.
Competition kinetics of defensins (10 μg [○]; 20 μg [×]) and different HLA-DR1-binding peptides (CLIP 80-104: LPKPPKPVSKMRMATPLLMQALPMG (□); IM 19-31: PLKAEIAQRLEDV (▪), and HA 306-18 (PKYVKQNTLKLAT ([▴]), each of them at a concentration of 30 μmol/L) to solubilized HLA-DR using a gel filtration assay; 1.5 μmol/L of AMCA-labeled HA 306-18 was used as a binding peptide.