The effect of TOR and IL-10 on the morphology of monocyte-derived DC.

(A) A macrophage-like appearance of adherent PBMC cultured in a medium for 7 days. (B) Immature DC cultured with IL-4 + GM-CSF for 7 days displaying dendrites and veils on the cell surface. (C) Mature DC cultured with IL-4 + GM-CSF followed by LPS (1 μg/mL) for 24 hours displaying a markedly dendritic surface morphology. (D) Cells cultured with IL-4 + GM-CSF and 5 μmol/L TOR or (E) IL-4 + GM-CSF and 10 μmol/L TOR with an intermediate morphology between macrophages and immature DC, with clearly fewer dendritic processes than immature DC. (F) Cells cultured with IL-4 + GM-CSF and IL-10 (100 U/mL) with a macrophage-like appearance. Magnification ×1000. The results are representative of 5 individual experiments.