Fig. 3.
![Fig. 3. TOR and IL-10 inhibit the function of monocyte-derived DC. / Adherent PBMC were cultured in a medium alone (control) or with IL-4 + GM-CSF in the presence or absence of TOR or IL-10 (100 U/mL). After 7 days of culture, the cells were washed, irradiated (30 Gy), and counted. Five to 20 × 103 cells were then cocultured with 2 × 105 allogeneic PBMC for 5 days, after which the proliferative activity was measured. The results are expressed as mean counts per minute ([cpm] ± SD) of a representative experiment made in triplicate. Similar results were obtained in another 2 experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/9/10.1182_blood.v95.9.2875.009k12_2875_2882/5/bloo00912003x.jpeg?Expires=1735857841&Signature=PKzVaexeUt8mvHcUXtp0oFufcJDl5ft5O4EWU~6efWGnqedNtto2h87wgnKUlYa~p6vfxp~9UTcqad-jsse7QnZY3VQ6iMLrqZl54NnQEEv-K8LqDtV0LE5aup1Z9KowOHCuFHdcFeQUtvsnXqigYKcrBXyUjZugXWqqkbq1k2kesKfby~JarGrRr2VrXHkfFKOOwprVK-sdP2lByYn2IpCrye4lLrse7J5sbS5minH2jOoL29m6wi4KqOzg1Gf~EdyhdRW7yFKZteDKZCYUq3GfQNt6o~rIBGY5aX7rq5J-mZ6uEeGgMdKTEe8Be3ui2uYcehjvl0eWWXeBgo7bpQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
TOR and IL-10 inhibit the function of monocyte-derived DC.
Adherent PBMC were cultured in a medium alone (control) or with IL-4 + GM-CSF in the presence or absence of TOR or IL-10 (100 U/mL). After 7 days of culture, the cells were washed, irradiated (30 Gy), and counted. Five to 20 × 103 cells were then cocultured with 2 × 105 allogeneic PBMC for 5 days, after which the proliferative activity was measured. The results are expressed as mean counts per minute ([cpm] ± SD) of a representative experiment made in triplicate. Similar results were obtained in another 2 experiments.
