TOR and TAM inhibit DC differentiation by mechanisms not involving the ER.

Adherent PBMC were cultured with (A) IL-4 + GM-CSF alone or in combination with (B) 5 μmol/L TOR, (C) 5 μmol/L TOR + 1 μmol/L E2, (D) 5 μmol/L TAM, or (E) 5 μmol/L TAM + 1 μmol/L ICI 182780. After 7 days of culture the cells were stained for CD1a expression and analyzed with flow cytometry. Representative histograms from 3 individual experiments are shown.