The effect of TOR and IL-10 on the kinetics of CD80 and CD86 expression on adherent PBMC cultured with IL-4 + GM-CSF.

Adherent PBMC were cultured in a medium alone (control) or with IL-4 + GM-CSF in the presence or absence of TOR or IL-10 (100 U/mL). After 1, 3, and 6 days of culture the cells were stained for (A) CD80 and (B) CD86 expression and analyzed with flow cytometry. The MFIRs (see “Materials and methods”) of a representative experiment from 3 are shown.