Effect of PD98059 on ERK and pERK localization in v-fes–overexpressing macrophages.

VFB cells were incubated for 16 to 18 hours in DMEM supplemented with 10% FBS and treated (B, D) or not (A, C) for 90 minutes with 30-μmol/L PD98059. Immunostaining was performed with α-ERK (A, B) or polyclonal α-pERK rabbit antibody (C, D), followed by Cy3-conjugated α-rabbit antibody.